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This volume highlights the potentials as well as the limits and challenges of human breath analysis and describes the current efforts made to advance this promising technology from bench to bed. Human breath analysis is a young, interdisciplinary and innovative research field aiming to provide a smart and non-invasive diagnostic tool, which can be used for screening, detecting and monitoring of diseases or metabolic disorders. This book presents different approaches for breath analysis including real-time and offline mass spectrometry as well as optical and semiconductor gas sensing methods. Besides, the role of smart algorithms to improve the performance of those technologies and the importance of pulmonary function diagnostics for more reliable and meaningful breath analysis are highlighted. Finally, current application scenarios and future perspectives of breath analysis and pulmonary functioning tests are addressed.The volume is useful for researchers, who are new in the field, to easily get an overview of the current status and the challenges present in human breath analysis. Topics from fundamental research over targeted sensor development and application scenarios are described. Thus, this volume covers all development stages providing support and inspiration for engineers, medical doctors and scientists from various fields.
This book provides a selection of recent developments in scanning ion conductance microscopy (SICM) technology and applications. In recent years, SICM has been applied in an ever-increasing number of areas in the bioanalytical sciences. SICM is based on an electrolyte-filled nanopipette with a nanometer-scale opening, over which an electric potential is applied. The induced ion current is measured, which allows to directly or indirectly quantify various physical quantities such as pipette-sample distance, ion concentration, sample elastic modulus among many others. This makes SICM well suited for applications in electrolytes - most prominently for the study of live cells.This book starts with a historic overview starting from the days of the invention of SICM by Paul Hansma at the University of California at Santa Barbara in 1989. SICM is a member of the family of scanning probe microscopies. It is related to another prominent member of the family, atomic force microscopy (AFM), which has found application in almost any field of nanoscale science. The advantages and disadvantages of SICM over AFM are also outlined. One of the most effective and break-through applications of SICM nanopipettes is in electrochemistry. The different routes and applications for doing electrochemistry using nanopipettes are also discussed. In addition the book highlights the ability of SICM for surface positioning with nanometer precision to open up new vistas in patch clamp measurements subcellular structures. Finally the book presents one research area where SICM has been making a lot of contributions, cardiac research and the endeavors to combine SICM with super-resolution optical microscopy for highest-resolution joint topography and functional imaging.
This book is dedicated to label-free, non-invasive monitoring of cell-based assays and it comprises the most widely applied techniques.
This book is dedicated to label-free, non-invasive monitoring of cell-based assays and it comprises the most widely applied techniques.
This volume offers a careful selection of trend-setting topics in the field. In-depth review articles illustrate current trends in the field. Experienced experts presenta comprehensive overview concerning the electrochemical biosensing of glucose for diabetes care from an industrial research and development perspectivea survey of bioassay applications for individually addressable electrochemical arrays, focusing on liquid-phase bioanalytical assaysa review of recent advances in the development of electronic tongues based on the use of biosensor arrays coupled with advanced chemometric data analysisnovel strategies of DNA biosensor development and corresponding applications for studies of DNA damagea survey of recent trends in the electrochemistry of redox proteins, including the increasing diversity of redox proteins used in electrochemical studies, novel immobilization strategies, and biosensor / biofuel cell applicationsan overview of electrochemical sensing of blood gases with advanced sensor conceptsa survey of recent bioelectroanalytical studies with high spatial resolution using scanning electrochemical microscopy with a wide range of applications covering imaging of living cells, studies of metabolic activity, imaging of local enzyme activity, and studies of transport through biolayersThis timely collection will be of interest not only for experts in the field, but also to students and their teachers in disciplines that include analytical chemistry, biology, electrochemistry, and various interdisciplinary research areas.
This book reviews several aspects of the biological response to nanoscale particles on a molecular and cellular level.
Expert authors provide critical, in-depth reviews of available methods for retrieving selective information out of complex biological systems. Coverage of this volume includes sensor development for the detection of small analytes, monitoring of biomolecular interactions, analysis of cellular function, development of diagnostic tools.
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