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Amino Acid Analysis: Methods and Protocols presents a broad spectrum of all available methods allowing for readers to choose the method that most suits their particular laboratory set-up and analytical needs.

Our understanding of the molecular and cellular mechanisms that control skeletal muscle development, regeneration, and adaptive responses to activity has increased dramatically in recent years, fostered by innovative techniques and approaches that are either specifically designed or adapted for research in skeletal muscle biology.

Over the past 40 years the field of molecular simulations has evolved from picosecond studies of isolated macromolecules in vacuum to studies of complex, chemically heterogeneous biological systems consisting of millions of atoms, with the simulation time scales spanning up to milliseconds.

Recent advances in genetics over the last quarter of a century, especially in molecular techniques, have dramatically reduced the cost of determining genetic markers and hence opened up a field of research that is increasingly helping to detect, prevent and/or cure many diseases that afflict humans.

Divided into five convenient sections, Rho GTPase: Methods and Protocols provides an historical overview of the field and an account of the phylogenetics of the Rho family, general biochemical methods, and functional assays that allow monitoring the consequences of manipulating Rho GTPases in a variety of contexts.

Metabolic engineering is the practice of genetically optimizing metabolic and regulatory networks within cells to increase production and/or recovery of certain substance from cells.

In Liver Stem Cells: Methods and Protocols, expert researchers focus on several hepatic progenitor cells, hepatic differentiation form stem cells, bile ductal cell formation from stem cells, liver stem cells and hepatocarcinogenesi, and application of liver stem cells for cell therapy.

Population genomics is a recently emerged discipline, which aims at understanding how evolutionary processes influence genetic variation across genomes.

The development of the hybridoma technology created the possibility to obtain unlimited amounts of monoclonal antibodies (mAb) with high specificity and affinity for any target and to introduce mAbs in a wide range of applications;

In Embryo Culture: Methods and Protocols, expert researchers in the field detail many of the methods which are now commonly used to study human embryo culture.

Today, cells are commonly analyzed en masse, with thousands of cells per sample, yielding results on the average response of the cells.

Transposable elements are used as genetic tools for dissecting the function of a specific gene and elaborating on mechanisms leading to genetic change and diversity, and the evolutionary impact of mobile DNA on the biology and evolution of organism.

The second edition of Bioinformatics and Drug Discovery has been completely updated to include topics that range from new technologies in target identification, genomic analysis, cheminformatics, protein analysis, and network or pathway analysis.

The detailed volume is focused on basic protocols for isolating antibodies and, at the same time, it selects a range of specific areas with the aim of providing guides for the overall process of antibody isolation and characterization as well as protocols for enhancing classical antibodies and antibody fragments.

Organogenesis entails the regulation of cell division, cell expansion, cell and tissue type differentiation, and patterning of the organ as a whole. It is essential to gain insight into how organs are initiated and how they develop. However, this very often is subject to technical difficulties as these processes take place embedded deep in tissues or are difficult to access or visualize. To achieve this, we need specialized techniques such as those concisely illustrated in Plant Organogenesis: Methods and Protocols. Chapters address topics such as how to study and image the structure of ovules and embryos of Arabidopsis thaliana, tools to establish cell lineages in order to visualize the contribution of each cell and cell division to the building of a mature organ, approaches to study the totipotency of several plant cells, techniques such as the use of fluorescence-activated cell sorting (FACS) to analyse transcriptomes and hormone levels in Arabidopsis, methods to investigate organogenesis in economically important crops, and computer-based approaches to bring everything together. Written in the successful Methods in Molecular Biology¿ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Plant Organogenesis: Methods and Protocols serves both professionals and novices with its well-honed methodologies in an effort to further our knowledge of this fascinating researchfield.

The second edition of p53 Protocols expands upon the first edition with progress in p53 research since the publication of the first edition.

A broad definition of a receptor is a specialized protein on or in a cell that recognizes and binds a specific ligand to undergo a conformational change, leading to a physiological response or change in cell function.

Plant Salt Tolerance: Methods and Protocols describes recent advances and techniques employed by researchers to understand the molecular and ionic basis of salinity tolerance and to investigate the mechanisms of salt stress perception and signalling in plants.

These include methods and techniques to identify regulatory RNAs, characterizing the function and expression of regulatory RNAs in bacterial cells, RNA structure prediction, and interactions between regulatory RNAs and proteins.