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A panel of internationally recognized research scientists and clinical investigators brings together a diverse collection of readily reproducible methods for identifying and quantifying a large number of specific genetic abnormalities associated with the broad spectrum of myeloid malignancies. Highlights include techniques for the detection of BCR-ABL mutations and resistance to imatinib mesylate, detection of the FIP1L1-PDGFRA fusion in idiopathic hypereosinophilic syndrome and chronic eosinophilic leukemia, classification of AML by DNA-oligonucleotide microarrays, and detection of the V617F JAK2 mutation in myeloproliferative disorders. In addition to gene rearrangments, other prognostically relevant molecular lesions such as FLT3 mutations and WT-1 overexpression are covered.

A cutting-edge collection of readily reproducible in vitro and in vivo methods to elucidate the mechanisms associated with cannabinoid function in health and disease.

The accurate and reliable diagnosis of transmissible diseases is the most powerful weapon available to ensure their control, and in some cases eradication. Indeed many viral infections display clinical signs that are indistinguishable from bacterial or parasitic infections.

Adenovirus Methods and Protocols, Second Edition, now in two volumes, is an essential resource for adenovirus (Ad) researchers beginning in the field, and an inspirational starting point for researchers looking to branch into new areas of Ad study.

Adenovirus Methods and Protocols, Second Edition, now in two volumes, is an essential resource for adenovirus (Ad) researchers beginning in the field, and an inspirational starting point for researchers looking to branch into new areas of Ad study.

Herpes Simplex Virus Protocols comprises a wide range of experimental protocols that should be especially useful to new workers in herpes virology. Obviously the range of topics covered cannot be comprehensive, but we have tried to provide protocols dealing with those procedures that are most widely used;

Helicobacter pylori Protocols offers an outstanding collection of state-of-the-art protocols for the identification and molecular manipulation of H. pylori, for the isolation and restriction endonuclease digestion of H. pylori chromosomal DNA, and for the transformation and insertional mutagenesis of H.

Vaccine research and development is advancing at an unprecedented pace, with an increasing emphasis on rational design based upon a fundamental und- standing of the underlying molecular mechanisms. The aim of this volume is to provide a selection of contemporary protocols that will be useful to both novice and advanced practitioner alike. The variety of procedures required to design, develop, produce, and assess a vaccine is immense and covers aspects of ch- istry, biochemistry, molecular biology, cell biology, and immunology. No single volume can hope to cover these topics exclusively. Rather, here we attempt to provide a methods sourcebook focusing on hands-on practical advice. Comp- mentary and background information may be found in other volumes in the Me- ods in Molecular Medicine series. Of particular interest are volumes on Dendritic Cell Protocols, Interleukin Protocols, Vaccine Adjuvants, and DNA Vaccines. Since the publication of the first edition of Vaccine Protocols there have been major advances, particularly in the areas of bacterial genomics, antig- specific T-cell quantification, genetic manipulation of vaccine vectors, the h- nessing of natural molecules concerned with the regulation of immune responses, and the burgeoning field of DNA vaccinology. Hence, the extensive revision of this edition with new chapters on live viral vaccine vectors, atte- ated bacterial vectors, immunomodulators, MHC-peptide tetrameric complexes, and the identification of vaccine candidates by genomic analysis. Additionally, chapters from the first edition have been updated to accommodate state-of-t- art methods in vaccinology.

This book applies modern molecular diagnostic techniques to the analysis of single cells, small numbers of cells, or cell extracts. Emphasis is placed on non-invasive analysis of single cell metabolites and the direct analysis of RNA and DNA from single cells, with a focus on polymerase chain reaction and fluorescence in situ hybridization.

Helicobacter pylori Protocols offers an outstanding collection of state-of-the-art protocols for the identification and molecular manipulation of H. pylori, for the isolation and restriction endonuclease digestion of H. pylori chromosomal DNA, and for the transformation and insertional mutagenesis of H.

Herpes Simplex Virus Protocols comprises a wide range of experimental protocols that should be especially useful to new workers in herpes virology. Obviously the range of topics covered cannot be comprehensive, but we have tried to provide protocols dealing with those procedures that are most widely used;

From the viewpoint of this volume, lung inflammation is now recognized as central to the pathophysiology of a number of severe respiratory conditions, the two most common being asthma and chronic obstructive pulmonary disease (COPD).

Comprehensive and up-to-date, the two volumes of Cartilage and Osteoarthritis provide researchers and bench scientists with readily reproducible protocols for new experiments to understand-from the cellular to the animal level-the pathophysiology of cartilage and to discover molecular targets for pharmacological intervention.

A thoroughly revised and updated collection readily reproducible techniques for culturing human cells. This new edition includes a wide range of human cell types relevant to human disease and new chapters on fibroblasts, Schwann cells, gastric and colonic epithelial cells, and parathyroid cells.

This work covers the applications of lectins in medical research by presenting overviews of their current use as well as detailed protocols and techniques for use by medical researchers utilizing lectins.

Leading experts in the use of MRI explain its basic principles and demonstrate its power to understand biological processes with numerous cutting-edge applications.

Pancreatic Cancer: Methods and Protocols provides a broad range of protocols for molecular, cellular, pathological, and statistical analyses of s- radic and familial pancreatic cancer.

In keeping with the organization used effectively in other volumes in this series, each chapter has a brief introduction followed by a list of required items, protocols, and notes designed to help the reader perform the expe- ments without difficulty.

Leading basic researchers and clinical scientists describe in detail a wide variety of established and cutting-edge techniques they have developed to study the lifecycle and biological properties of the human papillomavirus.

A state-of-the art collection of readily reproducible laboratory methods for assessing chemosensitivity in vitro and in vivo, and for assessing the parameters that modulate chemosensitivity in individual tumors.

Despite subsequent efforts focused on the epidemiology, cell biology, immunology, molecular biology, and clinical manifestations of malaria and the Plasmodium parasite, there is still no licensed vaccine for the prevention of malaria.

Harry Baker and Rosalind Ridley have done an admirable job in assem bling this collection of articles that describe the methodology frequently used to study a group of CNS illnesses often referred to as the "prion diseases."

The development of cancer is a slow process and it very often takes a number of years before a tumor becomes clinically evident.

Laboratory studies in hemostasis have traditionally focused on abn- malities of platelet function or the quantitative and qualitative disorders that affect the proteins involved in blood coagulation.

The last decade has witnessed a steady stream of new synthetic approaches to peptide mimetics, compounds that replace phy- ologically vulnerable peptide functionalities with chemical modules of - creased stability and cellular penetration.

In Drug Targeting: Strategies, Principles, and Applications we have tried to compile a state-of-the-art volume on current targeting approaches.

The aim of Hematologic Malignancies: Methods and Techniques is to review those methods most useful for the diagnosis and subsequent mana- ment of hematologic malignancies.

We are in an exciting era in the war against cancer, with real prospects for novel anticancer drugs that are cancer cell-specific without the toxicities that have been the hallmark of conventional cytotoxic cancer chemotherapy.

Suicide Gene Therapy: Methods and Reviews covers gene therapy that targets malignant cells in a treatment that has become known as "suicide gene therapy. Additional trials used multiple gene therapy protocols that also involved suicide gene therapy (83 with 497 patients), indicating that the interest in this area is considerable.

A collection of basic and advanced molecular methods that reveal those markers essential for diagnoses of specific diseases. The techniques range from in vitro methods to in vivo models of prostate cancer, and include new methods for the accurate diagnosis of prostate cancer, and proteome and microarray analyses.