Bøker i Methods in Signal Transduction Series-serien

Ion channels generate bioelectricity. This book reviews nonclassical ion channel research, ranging from the basic biology, structure, regulations to their functions not only in normal physiology but also neurological disorders, using a variety of cutting-edge techniques and novel animal models.

Presents an analysis of various lipid signaling pathways. This book presents fresh experimental tools to investigate novel lipid mediators in signal transduction. It examines the role of lipid mediators in signal transduction and cell-to-cell communication.

Optogenetics represents a breakthrough technology capable of dynamically modulating molecular and cellular activity in live cells with high precision. This transformative technology made it possible to reversibly interrogate protein actions, cellular events, and animal behaviors with a flash of light.

This book focuses on neuron signaling in the regulation of metabolism and body weight, and especially on methods used in these studies. Obesity and related metabolic syndromes have reached epidemic status, but still are no effective strategies for prevention and treatment. Body weight homeostasis is maintained by balanced food intake and energy expenditure, both of which are under the control of brain neurons. In the recent years, significant progress has been made in identifying specific neurons, neural pathways, and non-neuron cells in feeding regulation, as well as in delineating autonomic nervous systems targeting peripheral metabolic tissues in the regulation of energy expenditure and metabolism. This book reviews recent progress on important neuron signaling for body weight and metabolic regulation and the state-of-the-art methods that has been applied in this field, ranging from animal models with neuron-specific manipulations, pharmacology, optogenetics, in vivo Ca2+ imaging, and viral tracing. Readers will be exposed to latest research frontiers on neuron regulation of metabolism.Key FeaturesExplores the role signaling between neurons plays with respect to metabolismDocuments how neurotransmitters affect the regulation of feedingDescribes various methods and technologies used to study the neuronal control of metabolismIncludes contributions from an international team of leading researchers.Related TitlesLim, W. & B. Mayer. Cell Signaling: Principles and Mechanisms (ISBN 978-0-8153-4244-1)Feltz, A. Physiology of Neurons (ISBN 978-0-8153-4600-5)Zempleni, J. & K. Dakshinamurti, eds. Nutrients and Cell Signaling (ISBN 978-0367-39307-6)

Ion channels generate bioelectricity. This book reviews nonclassical ion channel research, ranging from the basic biology, structure, regulations to their functions not only in normal physiology but also neurological disorders, using a variety of cutting-edge techniques and novel animal models.

This book focuses on neuron signaling in the regulation of metabolism and body weight and associated methods. The book will cover recent progress on neuron signaling for body weight and metabolic regulation and the state-of-the-art methods. Readers will be exposed to latest research frontiers on neuron regulation of metabolism.

This book describes methods used to investigate the function and distribution of lipids in membranes. Contributors present discoveries that have to do with lipid analysis and lipid interactions with other components of membranes, such as proteins, receptors, transporters and ion channels.

This volume will describe a variety of methodologies employed for the study of epithelial cells. These methods include isolating primary cells and generate and maintain organoid cultures; culturing intestinal and renal epithelial cell lines; characterizing epithelial cell polarity and differentiation; and others.

Providing detailed methods for the study of transient receptor potential (TRP) channels and their role in a wide variety of cellular functions, this book includes expression and functional studies of TRP channels in heterologous systems as well as structural and functional studies of TRP channels. The author also explores various methods for detailed analysis of biophysical properties of TRP channels. In addition, the text covers high-throughput screening assays for TRP channels and offers novel and effective approaches to study endogenous TRP channel function in native systems.

Presents an analysis of various lipid signaling pathways. This book presents fresh experimental tools to investigate novel lipid mediators in signal transduction. It examines the role of lipid mediators in signal transduction and cell-to-cell communication.

T cells play a vital role mediating adaptive immunity, a specific acquired resistance to an infectious agent produced by the introduction of an antigen. There are a variety of T cell types with different functions. They are called T cells, because they are derived from the thymus gland. This volume discusses how T cells are regulated through the operation of signaling mechanisms. Topics covered include positive and negative selection, early events in T cell receptor engagement, and various T cell subsets.

This book aims to provide an up-to-date overview of the many signaling pathways regulating autophagy in response to different cellular needs. It explores the mechanisms and significance of cross-talk between autophagy and other cellular processes. Lastly, alterations in autophagy observed in aging and age-related pathologies is described.

Showcasing the recent progresses of the field, Cyclic Nucleotide Signaling covers the major tools and methodologies used in various areas of research.

Presents the structure of phototransduction cascade components at atomic resolution, as well as molecular interactions in multi-protein complexes and novel cell-based strategies for understanding signal shut-off and light adaptation. This book compares the visual signaling processes of vertebrates and invertebrates.

Signaling through cell adhesion molecules have long been of interest because of their importance in embryonic development, homeostasis, immune responses, wound healing , and malignant transformation. This book brings together various methodologies employed in the study of signaling by cell adhesion molecules.

Focusing on specific pathways or pathway components, this book provides the methods and guidelines for experimental design to study major aspects of cell signaling in vertebrate embryos. It covers a broad range of topics in signaling and a variety of current model organisms.

Calcium Entry Channels in Non-Excitable Cells focuses on methods of investigating the structure and function of non-voltage gated calcium channels. Each chapter presents important discoveries in calcium entry pathways, specifically dealing with the molecular identification of store-operated calcium channels which were reviewed by earlier volumes in the Methods in Signal Transduction series. Crystallographic and pharmacological approaches to the study of calcium channels of epithelial cells are also discussed. Calcium ion is a messenger in most cell types. Whereas voltage gated calcium channels have been studied extensively, the non-voltage gated calcium entry channel genes have only been identified relatively recently. The book will fill this important niche.

This volume is an extension of the subjects covered in the first edition. There are 5 sections each with 4 to 7 chapters, presenting technique advances for studyng mammalian lipds, analyzing plant lipids and signaling, and the impact of bioactive lipids on receptor function.