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This book provides the first dedicated overview of neurophotonics, covering the use of advanced optical technologies to record, stimulate, and control the activity of the brain, yielding new insight and advantages over conventional tools due to the adaptability and non-invasive nature of light.

This book explores the diverse capabilities of optical probes and presents various approaches used to design, develop, and implement these powerful and versatile tools. It examines the use of optical probes to detect and track numerous molecular processes in living cells, including GTPase and kinase activities, metabolic signals, RNA, and histone modifications. The book also covers sophisticated imaging techniques and equipment used to visualize and track optical probes and discusses emerging techniques that are expanding optical probe-based approaches into new biological frontiers.

This guide covers the current state of knowledge on intrinsic fluorescent biomarkers and highlights advances in using these biomarkers for the metabolic mapping and clinical diagnosis of healthy and diseased cells and tissues. The book¿s first section introduces the fundamentals of cellular energy metabolic pathways as well as natural biomarkers within the context of their biological functions. The second section outlines the theoretical and technical background of quantitative, noninvasive, autofluorescence microscopy and spectroscopy methods. The last two sections explore applications in biochemistry, cell biology, and medicine.

This book encompasses the full breadth of the super-resolution imaging field, representing modern techniques that exceed the traditional diffraction limit, thereby opening up new applications in biomedicine. It shows readers how to use the new tools to increase resolution in sub-nanometer-scale images of living cells and tissue, which leads to new information about molecules, pathways and dynamics. The book highlights the advantages and disadvantages of the techniques, and gives state-of-the-art examples of applications using microscopes currently available on the market.

This book explores the diverse capabilities of optical probes and presents various approaches used to design, develop, and implement these powerful and versatile tools. It examines the use of optical probes to detect and track numerous molecular processes in living cells, including GTPase and kinase activities, metabolic signals, RNA, and histone modifications. The book also covers sophisticated imaging techniques and equipment used to visualize and track optical probes and discusses emerging techniques that are expanding optical probe-based approaches into new biological frontiers.