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As former Boston cops, Drake Matthews and Alison Monroe thought they had experienced it all... until they found themselves tracking down a serial killer who turned out to be one of the undead. Stopping him cost them their careers and almost their lives. Thanks to an influential and anonymous benefactor, Drake and Alison find a new job ridding the streets of Washington D.C. of the vampiric threat terrorizing the nation's capital. Only this time, Drake and Alison are not facing a single vampire but an entire nest led by Ion Zielenska, one of history's most evil and twisted masters. As the vampires indiscriminately prey on humans, seeing them as nothing more than food to satiate their hunger, they create a wave of violence that threatens to engulf the city. Orchestrating the carnage is Antoinette Varela, the mistress of the nest, who realizes that for the nest to survive the hunters must be eliminated. However, when her vendetta turns personal, the hunters find themselves in struggle they are not prepared for.

Within the vaults of the Smithsonian Institute lies the key to finding the Vampyrnomicon, the Book of the Undead, that contains the history and secrets of the vampires. According to legend, whoever possesses the book can establish a vampire nation on earth - or destroy the undead once and for all. With an opportunity to end the war against the undead so close, Drake Matthews is determined to find the book. But the vampires also want The Vampyrnomicon. When Master Chiang Shih and her coven of the most powerful and dangerous vampires arrive in Washington to claim the book as their own, the hunters find themselves facing their most dangerous enemy yet. With the stakes so high, so is the ferocity of the struggle.