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This volume discusses methods used in the fields of molecular and cellular biology for detecting and studying cell death, especially in cancer and cancer therapy. Chapters in this book cover topics such as non-destructive, real-time Annexin V apoptosis assay; assessment of the immune response to tumor cell apoptosis and efferocytosis; mass cytometry assessment of cell phenotypes and signaling states in human whole blood; cell cycle analysis of ER stress and autophagy; and simultaneous detection of inflammasome activation and membrane damage during pyroptosis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Cutting-edge and practical, Apoptosis and Cancer: Methods and Protocols is a valuable resource and practical guide for both novice and expert researchers looking into the "e;meaning of death."e;
Tracker. The very word evokes images of buckskin-clad braves crouching over the ground, carefully studying the signs before them-a part of history. But the modern world has not put behind it the need for the earthy business of tracking. Such skills are still routinely used by the military, rescue personnel, and law enforcement, as well as by hunters and people living at subsistence level throughout the world. Tracking Humans is the ultimate authoritative guide to this most complex pursuit. A great resource for military, law enforcement, and rescue professionals, Tracking Humans is also useful for outdoor enthusiasts. Users will find it invaluable as an on-site manual to assist in any ongoing search.Unlike many tracking manuals, this guide focuses on tracking humans, whether they're enemy combatants or lost children. Author David Diaz explains what it takes to be an expert tracker, from the physical stamina to the focus and perception necessary to do the job correctly. He explains the tools of the tracker and presents essential safety tips every tracker should know. Tracking Humans is an important tool for anyone dealing with missing persons?it could be an essential lifesaver.
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