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In situ hybridization is a technique that allows for the visualization of specific DNA and RNA sequences in individual cells, and is an especially important method for studying nucleic acids in heterogeneous cell populations. in situ Hybridization in Electron Microscopy reviews the three main methods and describes the different stages in detail: th
In situ hybridization is a technique that allows for the visualization of specific DNA and RNA sequences in individual cells. This book reviews the three main methods and describes the different stages such as: the preparation of tissue, pretreatment, hybridization, and visualization of the hybridization products.
Covers methods of in situ polymerase chain reaction (PCR) and reverse transcription PCR (RT-PCR), two fresh approaches in visualizing very low amounts of DNA and mRNA in tissues and cell cultures at the light and electron microscope levels. This title provides theoretical consideration, as well as practical approaches to in situ PCR.
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