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Glycosaminoglycans: Chemistry and Biology emphasizes several areas of glycosaminoglycan research especially analysis and application of GAGs using a variety of approaches.

This book is an important new resource for clinicians caring for ventilator dependent children, who often have complex health care needs, are supported by advanced technology and are at high-risk of serious complications.

Reflecting a vast amount of new information concerning the functional characteristics of the various 5-HT receptor subtypes and the Na+-dependent serotonin transporter (SERT), this volume provides state of the art methodologies currently applied in serotonin research from leading experts in the field.

To foster a better understanding of dopamine receptor functionality, this detailed volume creates an interface between updated classical methods and new emerging technologies heretofore not available to new or seasoned researchers.

This volume contains collection of Natural Killer Cell methodologies relevant for both basic and translational research. These methodologies present new developments in the natural killer (NK) cell field, such as understanding the influence of NK cells metabolism on its function, identifying complexity of NK cell subsets through mass cytometry, and determining the emergence of memory NK cells in murine model of MCMV infection. Methods that study NK cell migration and cytotoxicity through endpoint analysis or live single cell imaging are also discussed. Chapters also describe methods pertaining to translational application of NK cells, such as ex vivo expansion of NK cells on K562 cell lines genetically modified to express either membrane bound IL-15 or membrane bound IL-21, large scale NK cell culture, current techniques for engineering NK cells to express chimeric antigen receptors or chemokine receptors using retroviral vectors,electroporation of mRNA, and the natural phenomenon of trogocytosis. Written in the highly successful Methods in Molecular Biology series format, these chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Cutting edge and thorough, Natural Killer Cells: Methods and Protocols is a valuable resource for researchers who not only want to understand mechanisms that govern NK cell behavior and diversity, but also for those who want to understand how to systematically evaluate NK cells for adoptive immunotherapy applications.

This new edition provides updated and novel protocols of neuroproteomics methods that encompass both global-scale as well as targeted and specialized topics, which are timely additions for the molecular and phenotypic analysis of the central nervous system and CNS-related disorders.

This second edition volume expands on the previous edition by discussing classic techniques, as well as new protocols that focus on the preparation of liposomes, lipid characterization, particle size and charge analysis, drug encapsulation, surface modification, stimuli response, and cellular interaction and biodistribution.

This volume presents classical approaches to in vivo neuropharmacology and neurophysiology, such as c-fos, electrochemistry, microdialysis microstimulation, and push-up superfusion. It also explores exciting new methods for behavioral analysis, and techniques based on optogenetics and non-invasive magnetic resonance imaging. The chapters of this book cover topics such as principles of stereotaxy, pharmaco-based fMRI and neurophysiology in humans and non-human primates, electrical nerve stimulation and central microstimulation, involvement of neurotransmitters in mnemonic processes, the impact of cannabinoids on motor activity, as well as the involvement of nitric oxide in neurotoxicity produced by psychostimulant drugs. Each chapter also discusses difficulties, tips, tricks, and precautions to take. Neuromethods series style chapters include the kind of detail and key advice from the specialists needed to get successful results in your own laboratory.Cutting-edge andpractical, In Vivo Neuropharmacology and Neurophysiology is a valuable resource for experienced and less experienced investigators of brain function and brain disorders.

The second edition of this volume provides up-to-date methods on the main methodological aspects of functional MRI (fMRI), applying fMRI to the study of central nervous system, and future evolutions of fMRI techniques.

Chapters focus on in-vivo recordings, voltage-gated channel recording and analysis, dendritic and axonal recordings, synaptic current recording and analysis, advanced fluorescent techniques, optogenetics and voltage-sensitive dye imaging, and finally channel and neuronal modeling.

This volume describes the most common laboratory procedures for isolation, identification and characterization of polioviruses used in clinical and research laboratories.

Electrophysiological methods are used at nearly all levels of biological research today. Dr. Yan Dong, an expert in utilizing electrophysiological techniques for brain research with fifteen years of experience, and Peter Neumann hope this work can act as a concise and practical aid to remedy the current situation.

This text provides a comprehensive, state-of-the-art review of the application of image analysis focusing on the techniques which can be used in every biology and medical laboratory to automate procedures of cell analysis and to create statistics very useful for a comprehension of cell growth dynamics and the effects of drugs on them.

Challenging Cases in Allergic and Immunologic Diseases of the Skin offers clinicians a wide range of challenging cases that are certain to provide hours of thoughtful and instructive review.

Bone Pathology is the second edition of the book, A Compendium of Skeletal Pathology that published 10 years ago. Similar to the prior edition, this book complements standard pathology texts and blends new but relatively established information on the molecular biology of the bone.

Providing a spectrum of models that is reflective of the various species that can be utilized in experimentation on disorders across a broad range of developmental disabilities, this volume collects expert contributions involved in investigation of the causes, outcomes, treatment, and prevention.

Asthma is a chronic relapsing airways disease that represents a major public health problemworldwide.

A comprehensive survey of nonsurgical treatment for a variety of heart diseases that affect the cardiac valves, the heart muscle, and the structure of the heart.

This book provides an overview of pulmonary hypertensive diseases, the current understanding of their pathobiology, and a contemporary approach to diagnosis and treatment.

This detailed volume explores key technologies that are used currently to investigate iGluR structure, function and physiology.

Viral Vector Approaches in Neurobiology and Brain Diseases

As such, many techniques are required to understand the contribution and functions of neuropeptides in health and disease processes. Research in the neuropeptide field is aimed toward a better understanding of the physiology of the human body and toward the development of better diagnostics and therapeutics.

This comprehensive contemporary survey of the non-motor facets of Parkinson's disease has now been fully updated and expanded for a new edition that covers all the autonomic, sensorimotor, sleep-related, and sensory aspects of non-motor dysfunction.