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Plant Cell and Tissue Culture continues the high standards of Humana's Methods in Molecular Biology series. Its step-by-step approach (a hallmark of the series) is applied to a wide range of basic laboratory techniques and culture conditions appropriate to plant cells.Because of the diversity of cell types, species, and culture methods, much of this volume is devoted to the culture of particular cell types and to the regeneration of these cells into whole plants. Special attention is also given to the genetic modification of plants, as well as to the economic significance of plant products. Chapters cover a wide range of topics and techniques, including:. tissue culture media and selection . cryopreservation . callus culture techniques . organ culture . embryogenesis . batch culture . large-scale culture . hormonal control . fertilization techniques . gene transfer . cell immobilization . production systems . cell product purification . DNA expression . electrofusion of plant cells . mutant selection . mutagenesis techniques . automation . transfer of nuclei . protoplast culture . media analysis . micropropagation.A detailed appendix lists the formulas for the most commonly employed plant cell media. Comprehensive, easy to follow, and a pleasure to use, Pollard and Walker's Plant Cell and Tissue Culture is an essential tool for everyone--at all levels of proficiency and experience--involved in plant culture.

Thus, the function of the brain as a coordinator of vital homeostatic reflexes, and complex body reactions to external challenges, depends critically on the rate of oxygen delivery and oxygen consumption.

In ALLOCATING HEALTH CARE RESOURCES, leading authorities and researchers expose the basic philosophical, ethical, and economic issues underlying the current health care debate.

Now going into its third much-expanded edition, the highly praised Nutritional Health: Strategies for Disease Prevention has been brought fully up to date to include all the new thinking and discoveries that have the greatest capacity to improve human health and nutritional advancement.

Covering new developments in cellular, viral and non-biological therapies, and with chapters by experts from a range of disciplines, this is an up-to-date survey of the role that circadian rhythms, cardiac plasticity, and mechanotransduction play in the heart.

Targeted Therapies for Solid Tumors

This work, Lung Cancer, Volume 2: Diagnostic and Therapeutic Methods and Reviews, in the Methods in Molecular Medicine series presents an overview of the current status of those methods useful in the diagnosis and tre- ment of lung cancer-both as it exists in the clinic and as it is being revoluti- ized in the laboratory.

The study of gene regulation has been made more tractable by the design of simple expe- mental models in which a single gene can be isolated from the milieu of the organism. In - sence, the genome of the organism is dissected in such a manner that specific genes may now be introduced into an appropriate cell line .

The identification of hepatitis C virus by Michael Houghton and his c- leagues at the Chiron Corporation nearly years ago represented a technical tour de force of modern molecular medicine.

From the viewpoint of this volume, lung inflammation is now recognized as central to the pathophysiology of a number of severe respiratory conditions, the two most common being asthma and chronic obstructive pulmonary disease (COPD).

Computers have revolutionized the analysis of sequencing data. The following chap ters describe the process of aligning multiple sequences in order to assemble overlapping fragments into sequence contigs to compare similar sequences from different sources.

Laboratory studies in hemostasis have traditionally focused on abn- malities of platelet function or the quantitative and qualitative disorders that affect the proteins involved in blood coagulation.

Colin Graham and a team of leading investigators and expert clinical scientists update the acclaimed first edition with a collection of powerful, up-to-date PCR-based methods for DNA sequencing, many suitable for human genome sequencing and mutation detection in human disease.

Since the first recognition of outbreaks of cerebrospinal or spotted fever at the end of the nineteenth and the beginning of the twentieth centuries, the menace of the meningococcus has been high on the list of public health prio- ties.

In Drug Targeting: Strategies, Principles, and Applications we have tried to compile a state-of-the-art volume on current targeting approaches.

Biochemically, the overall process can be thought of as a series of steps in a reaction pathway whereby DNA molecules are brought into homologous register, the four-stranded Holliday structure intermediate is formed, hete- duplex DNA is extended, and DNA strands are exchanged.

give warnings, disclaimers, and the like.* The main objective of Protein and Peptide Analysis by Mass Spec trometry is quite straightforward-to present authoritative, up-to-date, and practical accounts of the use of mass spectrometry in the analysis of pep tides and proteins.

The development of recombinant DNA technology and methods for transferring recombinant genes into plants has brought about significant advances in plant science.

Here, as with enzymatic DNA sequencing, is a very simple concept that relies on minimal information to prepare short oligonucleotide primers that direct the synthesis of a specified fi-agment o f DNA in the presence of a thermostable DNA polymerase.

Harry Baker and Rosalind Ridley have done an admirable job in assem bling this collection of articles that describe the methodology frequently used to study a group of CNS illnesses often referred to as the "prion diseases."

In Chapter 1, Dokal and colleagues present simple protocols for the molecular investigation of Fanconi anemia and dyskeratosis congenita. In Chapter 3, Old details protocols for the molecular diagnosis of most forms of hemoglobinopathy.

The Methods in Molecular Medicine series is intended as a resource for both novice and experienced investigators attempting to diversify their tech- logical base in research.

Psychiatric Genetics provides the reader with a complete view of the methodological problems encountered in psychiatry genetics and proposes solutions to commonly occurring questions.

Minimally invasive urologic surgery is revolutionizing how physicians treat many urologic diseases. Laparoscopic Urologic Oncology focuses on educating both general urologists and urologic oncologists on the current and future role of laparoscopy and other minimally invasive techniques in urologic oncology.

In Buprenorphine Therapy of Opiate Addiction, participating physicians and toxicologists summarize and evaluate their experiences with five years of intensive buprenorphine therapy.

The rapid growth in the number of options available for the management of colorectal cancer presents the clinician with new opportunities and new complexities.

Internationally recognized investigators review the latest developments in, and novel approaches to, understanding the prion protein and prion diseases at the molecular level.