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The zebrafish has become an important model organism to study biologial processes in vivo. Among the topics discussed in this book are technical advances which allow the rapid testing of in vivo function of as yet uncharacterized genes in zebrafish in large numbers and at a great speed.

This book provides practical guidance for conducting topical research in fungal biology. It is particularly valuable in that it brings together highly diverse laboratory methods, covering genetics, molecular biology, biochemistry and microscopy, into a single volume.

Designed to enable researchers to design and undertake a phage display project, from designing an experimental strategy and constructing a library to performing selections and analyzing the results. The protocols and chapters in this book are cross-referenced, allowing readers to move beyond the specific examples provided.

The protein purification Practical Approach books contain detailed practical information on the separation and purification of proteins. Protein Purification Techniques covers unit operations and analytical techniques in detail, while Protein Purification Applications has details of protein purification from a selected number of typical sources.

Many investigations into the structure and function of cells and tissues require the isolation of a particular membrane or subcellular component (organelle). This book covers all the necessary aspects, from breaking up the cells (homogenization), via a variety of separation techniques (the isolation and fractionation chapters), to characterization of the separated organelles.

Pregnancy tests, HIV tests, and tests to confirm heart attacks and many other clinical diagnostic tests are all immunoassays. Without immunoassays a huge variety of biologically important molecules could not be easily detected or quantified. Immunoassays will help any life scientist develop his/her own immunoassay.

The three-dimensional structure of proteins is a key factor in their biological activity. There is an increasing need to be able to predict the structure of a protein once its amino-acid sequence is known; this book presents practical methods of achieving that ambitious aim, using the latest computer modelling algorithms.

Macrophages are an essential part of the immune response against antigens. This book contains methods for their isolation, purification, and analysis of their activity.

The first of a 2-volume set which provides a comprehensive handbook for the applications of molecular as well as classical techniques to plant pathology. Detailed protocols are included which address a range of investigations, from plant pathogen isolation to localizing genes and their products.

This is a concise yet comprehensive practical manual, enabling all those involved in the diagnosis of disease, caused by parasites, to provide both a competent and up-to-date service. There is also sufficient information on the biology of parasites provided to ensure that the book is a valuable resource for educational courses teaching parasitology in both the developed and devloping World.

Recombinant DNA techniques have revolutionized the isolation and production of antibodies in recent years, and rapid changes have been made. The time is now ripe to assemble in a Practical Approach volume the protocols which allow the researcher to isolate a new antibody, analyse its properties, format the right antibody molecule or fragment, and produce it in usable quantitites.

This book brings together methods for preparing and maintaining cell cultures from a diverse range of neural tissues and cells, from both the central and peripheral nervous systems.

Medical virology has undergone a revolution of diagnostic and scientific approaches through the advent of molecular biological techniques. This is reflected in the topics collected in this volume, which present classical and molecular techniques currently used for the diagnosis of viral infections.

This book is a guide for the newcomer to all those areas which are currently basic to the performance of animal (including human) cell culture. Experienced workers should also find it useful as a source of reference when entering a new field, or when setting up a new laboratory.

Acquired chromosome abnormalities are associated with cancer and leukaemia, exposure to some chemicals and radiation and some rare diseases. This volume is a laboratory manual of techniques for the cytogenetic study of these disorders.

Cytogenetics is concerned with matching inherited traits with the appearance, structure, and behaviour of chromosomes. Human Cytogenetics: Constitutional Analysis covers all aspects of basic human cytogenetic study other than malignancies and acquired abnormalities. They are covered in a separate volume.

The involvement of carbohydrates in biological processes has greatly fuelled the current interest in this diverse range of molecules. This book presents the up-to-date techniques required to analyse a wide variety of carbohydrates and carbohydrate-containing molecules.

Enzyme assays are among the most frequently performed procedures in biochemistry, routinely used (for example) to estimate the amount of enzyme present in a cell or tissue. This new edition describes the design and execution of enzyme assays, covering both general principles and specific examples.

This book is an up-to-date and unique collection of experimental protocols from an area of pharmaceutical research that is essential for the development of new, highly specific drugs as well as for the exploration of completely new therapeutic approaches to disease treatments.

Protein Structure: A Practical Approach, 2nd edition, gives general methods for determining protein structure, from the electrophoretic, chromatographic, and spectrophotometric techniques to the methods of mass spectrometry and ultracentrifugation.

Part of a four-volume set on DNA cloning, this volume covers the main expression systems in current use. It provides background information and advice on the merits of each, step-by-step practical protocols, and details of the latest applications.

Neurochemistry is the study of the cellular and subcellular components of the nervous system and the molecules found within it. This volume contains the core elements of neurochemistry that should be useful to both first time researchers and established investigators in all fields of neuroscience.

This book is one of four which together are considerably updated and totally revised texts that arose out of the very successful "DNA Cloning, Volume I-IV" books in the Practical Approach series. This particular book describes all the necessary background and detailed laboratory methods for the core techniques used in every molecular biology laboratory.

This text presents the best methods, hints and tips for core procedures such as one-dimensional polyacrylamide gel electrophoresis, isoelectric focusing, two-dimensional gel electrophoresis, preparative gel electrophoresis, and peptide mapping, with refinements and updates of the procedures.

A guide to frequently used procedures in clinical microbiology laboratories. This book is a useful training aid for the diagnostic microbiology laboratory, and is also suitable for microbiologists preparing for higher professional examinations such as membership of the Royal College of Pathologists and the American Board of Medical Microbiology.

Flow cytometry is a technique widely used in biological research and in diagnostic medicine. Flow cytometers are found in most biological research institutions and most clinical laboratories in larger hospitals.