Cryopreservation and Freeze-Drying Protocols

Part I: Fundamental Aspects of Cryopreservation and Freeze-Drying

1. Principles Underlying Cryopreservation and Freeze-Drying of Cells and Tissues
Willem F. Wolkers and Harriëtte Oldenhof

2. Principles of Ice-Free Cryopreservation by Vitrification
Gregory M. Fahy and Brian Wowk

3. The Principles of Freeze-Drying and Application of Analytical Technologies
Kevin R. Ward and Paul Matejtschuk

4. Mathematical Modeling and Optimization of Cryopreservation in Single Cells
James D. Benson

5. Mathematical Modeling of Protectant Transport in Tissues
Ross M. Warner and Adam Z. Higgins

Part II: Technologies and Methods to Study Freezing and Drying

6. Freezing Technology: Control of Freezing, Thawing, and Ice Nucleation
Peter Kilbride and Julie Meneghel

7. Microwave- and Laser-Assisted Drying for the Anhydrous Preservation of Biologics
Shangping Wang, Susan Trammell, and Gloria D. Elliott

8. High-Speed Video Cryomicroscopy for Measurement of Intracellular Ice Formation Kinetics
Jens O.M. Karlsson

9. Use of Ice Recrystallization Inhibition Assays to Screen for Compounds that Inhibit Ice Recrystallization
Anna A. Ampaw, August Sibthorpe, and Robert N. Ben

10. DSC Analysis of Thermophysical Properties for Biomaterials and Formulations
Wendell Q. Sun

11. Osmometric Measurements of Cryoprotective Agent Permeation into Tissues
Kezhou Wu, Leila Laouar, Nadia Shardt, Janet A.W. Elliott, and Nadr M. Jomha

12. Use of X-Ray Computed Tomography for Monitoring Tissue Permeation Processes
Ariadna Corral, Alberto Olmo, and Ram├│n Risco

13. Use of In Situ Fourier Transform Infrared Spectroscopy in Cryobiological Research
Willem F. Wolkers and Harriëtte Oldenhof

14. Raman Cryomicroscopic Imaging and Sample Holder for Spectroscopic Subzero Temperature Measurements
Guanglin Yu, Rui Li, and Allison Hubel

Part III: Cryopreservation and Freeze-Drying Protocols

15. Cryopreservation of Semen from Domestic Livestock: Bovine, Equine, and Porcine Sperm
Harriëtte Oldenhof, Willem F. Wolkers, and Harald Sieme

16. Cryopreservation of Avian Semen
Henri Woelders

17. Cryopreservation of Mouse Sperm for Genome Banking
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