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This up-to-date edition alternates scientific and clinical chapters that explain the basic science underlying neurological processes and then relates that science to the understanding of neurological disorders and their treatment.

This volume details protocols that cover the broad arsenal of techniques used to study a secretion system from A to Z. Chapters focus on identifying and localizing the different subunits, defining interactions within subunits, monitoring conformational changes, purifying and imaging of large complexes, defining the assembly pathway by fluorescence microscopy and the role of energy during assembly and/or secretion, identifying secreted effectors as well as reporters to follow effector transport. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge, Bacterial Protein Secretion Systems: Methods and Protocol aims to provide techniques that are not restricted to the study of secretion systems but arealso of specific interest for any researcher interested on multi-protein complexes of the bacterial cell envelope.

This volume provides key methods and protocols from laboratories engaged in germinal centers (GC) research with the expectation of stimulating further research, and to aid scientists in the study of GC biology and pathology.

This volume explores both simple and sophisticated techniques used in the study of different types of lateralization of brain and behavior. Part II covers neurobiological methods used to reveal lateralization, such as lesion studies, electrophysiology and pharmacology, early gene expression, and new optogenetic methods.

This fully updated and revised second edition address specifically some of the significant advances made in our understanding of the pathophysiology of penile cancer including discussion of the suspected role of the human papilloma virus in penile carcinogenesis.

Plant-Pathogen Interactions: Methods and Protocols, Second Edition expands upon the first edition with current, detailed protocols for the study of plant pathogen genome sequences.

This volume describes the methods of both in vivo and in vitro electroporation using ferrets, rats, mice, chickens, and zebrafish.

Biomedical Literature Mining, discusses the multiple facets of modern biomedical literature mining and its many applications in genomics and systems biology.

While there are Pediatric Urology texts for Pediatric Urologists and for General Urologists, there are none for Primary Care Physicians. Pediatric Urology for the Primary Care Physician will significantly help our primary care colleagues in the evaluation and management of children with genito-urinary problems.

Textbook of Personalized Medicine

Cardiac Tissue Engineering: Methods and Protocols presents a collection of protocols on cardiac tissue engineering from pioneering and leading researchers around the globe.

As part of the Current Clinical Oncology series, Lymphoma: Diagnosis and Treatment will be of value to medical oncologists, hematologists, radiation oncologists, and all physicians involved in the care of patients with lymphoid malignancies.

The field of adipose tissue biology has been expanding at a very rapid pace in the last few years. The volume is divided in four sections: the first two deal with basic adipose tissue and adipokine biology, while the last two address the problem of obesity and alterations in adipose tissue function from an epidemiological and clinical standpoint.

Here is a concise review of urgent and emergent urological conditions, covering diagnostic and therapeutic considerations in traumatic, infectious, obstructive, hemorrhagic, iatrogenic, vascular and congenital urological emergencies in adults and children.

The origin of modern intensive care units (ICUs) has frequently been attributed to the widespread provision of mechanical ventilation within dedicated hospital areas during the 1952 Copenhagen polio epidemic.

Transcranial Magnetic Stimulation seeks to enable new and existing practitioners to learn and follow established TMS protocols.