Listeria Monocytogenes

Part I: Detection, Quantification, and Confirmation

1. Traditional Methods of Analysis for Listeria monocytogenes
Rui Magalhães, Cristina Mena, Vânia Ferreira, Gonçalo Almeida, Joana Silva, and Paula Teixeira

2. MALDI-ToF MS: A Rapid Methodology for Identifying and Subtyping Listeria monocytogenes
Snehal R. Jadhav, Rohan M. Shah, and Enzo A. Palombo

3. Sample Preparation for qPCR Detection of Listeria from Food
Patrick Mester, Anna Kristina Witte, and Peter Rossmanith

4. qPCR Validation on the Basis of the Listeria monocytogenes prfA Assay
Anna Kristina Witte, Patrick Mester, and Peter Rossmanith

Part II: Subtyping Approaches

5. Serotype Assignment by Sero-Agglutination, ELISA, and PCR
Lisa Gorski

6. Pulsed-Field Gel Electrophoresis (PFGE) Analysis of Listeria monocytogenes
Karen Hunt and Kieran Jordan

7. Multi-Locus Sequence Typing (MLST) and Whole Genome Sequencing (WGS) of Listeria monocytogenes and Listeria innocua
Beatrix Stessl, Martin Wagner, and Werner Ruppitsch

Part III: Genotypic and Phenotypic Characterization

8. High-Throughput Characterization of Listeria monocytogenes Using the OmniLog Phenotypic Microarray
Laura Luque-Sastre, Kieran Jordan, Séamus Fanning, and Edward M. Fox

9. High-Throughput Screening of Biofilm Formation of Listeria monocytogenes on Stainless Steel Coupons Using a 96-Well Plate Format
Jessica A. Gray, P. Scott Chandry, John P. Bowman, and Edward M. Fox

10. Confocal Laser Microscopy Analysis of Listeria monocytogenes Biofilms and Spatially Organized Communities
Maud Darsonval, Marina Grégoire, Julien Deschamps, and Romain Briandet

11. Extraction and Preparation of Listeria monocytogenes Subproteomes for Mass Spectrometry Analysis
Tiago Santos and Michel Hébraud

Part IV: Strain Manipulation

12. Extraction and Analysis of Plasmid DNA from Listeria monocytogenes
Aidan Casey and Olivia McAuliffe

13. Generation of Nonpolar Deletion Mutants in Listeria monocytogenes Using the "SOEing" Method
Kathrin Rychli, Eva Harter, Caitriona M. Guinane, Karen Daly, Colin Hill, and Paul D. Cotter

14. Mutant Construction and Integration Vector-Mediated Genetic Complementation in Listeria monocytogenes
Cameron Parsons, Reha Azizoglu, Driss Elhanafi, and Sophia Kathariou